atp assay kit Search Results


atp assay kit luminescence  (Dojindo Labs)
95
Dojindo Labs atp assay kit luminescence
Atp Assay Kit Luminescence, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp assay kit  (MedChemExpress)
94
MedChemExpress atp assay kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atp Assay Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell adp atp ratio  (Dojindo Labs)
94
Dojindo Labs cell adp atp ratio
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Cell Adp Atp Ratio, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp chemiluminescence assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology atp chemiluminescence assay kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atp Chemiluminescence Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complex v  (Elabscience Biotechnology)
93
Elabscience Biotechnology complex v
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Complex V, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology atp assay kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atp Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell mitochondrial complex v  (Elabscience Biotechnology)
93
Elabscience Biotechnology cell mitochondrial complex v
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Cell Mitochondrial Complex V, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atplite kit  (Revvity)
91
Revvity atplite kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atplite Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp adp ratio chemiluminescence assay kit  (Elabscience Biotechnology)
94
Elabscience Biotechnology atp adp ratio chemiluminescence assay kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atp Adp Ratio Chemiluminescence Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp colorimetric assay kit  (Novus Biologicals)
90
Novus Biologicals atp colorimetric assay kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atp Colorimetric Assay Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell a tp viability detection kit  (MedChemExpress)
94
MedChemExpress cell a tp viability detection kit
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Cell A Tp Viability Detection Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp glo bioluminometric cell viability assay  (Biotium)
94
Biotium atp glo bioluminometric cell viability assay
PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, <t>ATP</t> <t>level,</t> <t>SOD</t> expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.
Atp Glo Bioluminometric Cell Viability Assay, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, ATP level, SOD expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.

Journal: Redox Biology

Article Title: Alpha-ketoglutarate accelerates granulocyte-monocyte progenitor differentiation and atherosclerotic plaque inflammation via oxoglutarate receptor 1

doi: 10.1016/j.redox.2026.104134

Figure Lengend Snippet: PNP perturbed redox and mitochondrial homeostasis. ( A–G ) Lineage −/low cells were treated with 0 or 0.1 U/mL PNP for 24 h. NAD expression, NMN expression, ATP level, SOD expression and activity, GPX activity, and catalase activity were measured by ELISA. ( H–J ) In parallel, the cells were harvested and incubated with H2DCFDA for 15 min to study the level of ROS by FACS. (K–P) To assess mitochondrial function, lineage −/low cells were treated with PNP for PBS or 24 h. After harvest, 3 × 10 5 cells were loaded for oxygen consumption using a Seahorse Bioscience XF96 extracellular flux analyzer. Two experimental groups were compared using the unpaired two-tailed Student's t-test. In the experiments involving ≥3 groups, one-way analysis of variance followed by Dunnett's test was used.

Article Snippet: Protein was extracted from PNP-treated lineage −/low cells to determine the expression of ribose-5-phosphate, IMPDH2, NAD, SOD, and ATP by ELISA, following the manufacturer's instructions (mouse IMPDH2 ELISA kit [#ml601123], mouse NAD ELISA kit [# JK691233 ], Enzyme-linked Biotechnology, China; mouse SOD ELISA kit [#CSB- E08556 m], Huamei Bioengineering, China; ATP Assay Kit [#HY-K0314], MedChemExpress, US).

Techniques: Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Incubation, Two Tailed Test

Correlation of LDL-c and TCA metabolites in human plasma samples. (A-B) The fasting plasma levels of LDL-c and isocitrate were determined in healthy human subjects (n = 40) and their correlation was analyzed by Spearman's correlation analysis. ATP: adenosine triphosphate; ELISA: enzyme-linked immunosorbent assay; GPX: glutathione peroxidase; LDL-c: low-density lipoprotein cholesterol; NAD: nicotinamide adenine dinucleotide; PNP: purine nucleoside phosphorylase; ROS: reactive oxygen species; SOD: superoxide dismutase. (C) A proposed model of HFD-induced skewed myelopoiesis and atherosclerosis via α-ketoglutarate. In mice fed a HFD, isocitrate was elevated and LDL-c increased isocitrate dehydrogenase expression, leading to enhanced α-ketoglutarate production. Acting through OXGR1, α-ketoglutarate upregulated PNP mRNA and protein expression. PNP catabolized guanosine/deoxyguanosine and inosine/deoxyinosine, generating ribose-1-phosphate. By interconversion, ribose-5-phosphate production was increased and initiated de novo purine biosynthesis, resulting in IMPDH2 production and GMP proliferation. PNP metabolized nicotinamide riboside to Nam, decreasing NMN and NAD production. PNP increased NAD kinase expression to further accelerate NAD consumption. Furthermore, PNP increased inflammatory protein expression such as NF-κB. All of which reduced NAD production promoted ROS production and inflammation to facilitate GMP differentiation. Ultimately, the pronounced GMP proliferation and differentiation led to inflammatory myeloid cell production and atherosclerotic progression. GMP: granulocyte-monocyte progenitor; HFD: high-fat diet; IMPDH2: inosine-5′-monophosphate dehydrogenase 2; LDL-c: low-density lipoprotein cholesterol; NAD: nicotinamide adenine dinucleotide; OXGR1: oxoglutarate receptor 1; PNP: purine nucleoside phosphorylase; ROS: reactive oxygen species.

Journal: Redox Biology

Article Title: Alpha-ketoglutarate accelerates granulocyte-monocyte progenitor differentiation and atherosclerotic plaque inflammation via oxoglutarate receptor 1

doi: 10.1016/j.redox.2026.104134

Figure Lengend Snippet: Correlation of LDL-c and TCA metabolites in human plasma samples. (A-B) The fasting plasma levels of LDL-c and isocitrate were determined in healthy human subjects (n = 40) and their correlation was analyzed by Spearman's correlation analysis. ATP: adenosine triphosphate; ELISA: enzyme-linked immunosorbent assay; GPX: glutathione peroxidase; LDL-c: low-density lipoprotein cholesterol; NAD: nicotinamide adenine dinucleotide; PNP: purine nucleoside phosphorylase; ROS: reactive oxygen species; SOD: superoxide dismutase. (C) A proposed model of HFD-induced skewed myelopoiesis and atherosclerosis via α-ketoglutarate. In mice fed a HFD, isocitrate was elevated and LDL-c increased isocitrate dehydrogenase expression, leading to enhanced α-ketoglutarate production. Acting through OXGR1, α-ketoglutarate upregulated PNP mRNA and protein expression. PNP catabolized guanosine/deoxyguanosine and inosine/deoxyinosine, generating ribose-1-phosphate. By interconversion, ribose-5-phosphate production was increased and initiated de novo purine biosynthesis, resulting in IMPDH2 production and GMP proliferation. PNP metabolized nicotinamide riboside to Nam, decreasing NMN and NAD production. PNP increased NAD kinase expression to further accelerate NAD consumption. Furthermore, PNP increased inflammatory protein expression such as NF-κB. All of which reduced NAD production promoted ROS production and inflammation to facilitate GMP differentiation. Ultimately, the pronounced GMP proliferation and differentiation led to inflammatory myeloid cell production and atherosclerotic progression. GMP: granulocyte-monocyte progenitor; HFD: high-fat diet; IMPDH2: inosine-5′-monophosphate dehydrogenase 2; LDL-c: low-density lipoprotein cholesterol; NAD: nicotinamide adenine dinucleotide; OXGR1: oxoglutarate receptor 1; PNP: purine nucleoside phosphorylase; ROS: reactive oxygen species.

Article Snippet: Protein was extracted from PNP-treated lineage −/low cells to determine the expression of ribose-5-phosphate, IMPDH2, NAD, SOD, and ATP by ELISA, following the manufacturer's instructions (mouse IMPDH2 ELISA kit [#ml601123], mouse NAD ELISA kit [# JK691233 ], Enzyme-linked Biotechnology, China; mouse SOD ELISA kit [#CSB- E08556 m], Huamei Bioengineering, China; ATP Assay Kit [#HY-K0314], MedChemExpress, US).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing